Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2002

Идентификатор DOI: 10.1016/S1011-1344(02)00360-3

Ключевые слова: bioluminescence; electron-excited states; energy transfer

Аннотация: The hypothesis of activity of the upper electron-excited states of the bacterial bioluminescent emitter was verified using dye molecules as foreign energy acceptors. Six compounds were selected having fluorescent state energies ranging from 25 700 to 32 000 cm(-1) (anthracene, pyrene, 1.4-bis(5-phenyloxasol-2-yl)benzene (POPOP), p-bis(o-methylstyryl)benzene (MSB), 2-methoxy-naphtalene, p-terphenyl), exceeding that of the bioluminescent emitter (22 000 cm(-1)). Their absorption spectra do not overlap with the bioluminescence spectrum; the trivial light absorption and the intermolecular resonance S-S energy transfer were excluded. Bacterial bioluminescent spectra of the coupled enzyme system NADH:FMN-oxidoreductase-luciferase in the presence of MSB were presented as an example. The weak sensitized fluorescence of MSB was registered. The results obtained have confirmed the activity of the energetic precursor in the bacterial bioluminescence. Its energy can be located in the interval of 26 000-27 000 cm(-1). (C) 2002 Published by Elsevier Science B.V.

Журнал: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY

Выпуск журнала: Vol. 68, Is. 2-3

Номера страниц: 88-92

ISSN журнала: 10111344

Место издания: LAUSANNE

Издатель: ELSEVIER SCIENCE SA

• Kudryasheva N.S. (Institute of Biophysics,Siberian Branch of Russian Academy)
• Nemtseva E.V. (Institute of Biophysics,Siberian Branch of Russian Academy)
• Sizykh A.G. (Krasnoyarsk State University)
• Kratasyuk V.A. (Krasnoyarsk State University)
• Visser A.J.W.G. (MicroSpectroscopy Center,Department of Biomolecular Sciences,Wagenigen University)

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