Сибирский федеральный университет

Год издания: 2020

Идентификатор DOI: 10.1038/s41597-020-00576-1

Адрес в архиве электронных ресурсов СФУ: https://elib.sfu-kras.ru/handle/2311/137957

Аннотация: Coast redwood is a very important endemic conifer timber species in Southern Oregon and Northern California in the USA. Due to its good wood properties and fast growth rate it can be considered as a prospective timber species also in other countries with similar or changing toward similar climatic conditions due to global climate warming, such as Germany. In general, it is frost sensitive and suffers from freezing temperatures. To study genetic mechanisms of frost resistance in this species and to select the most frost tolerant trees we tested 17 clones in climate control chamber experiments and generated two de novo assemblies of the coast redwood transcriptome from a pooled RNA sample using Trinity and CLC Genomic Workbench software, respectively. The hexaploid nature of the coast redwood genome makes it very challenging to successfully assemble and annotate the coast redwood transcriptome. The de novo transcriptome assembly generated by Trinity and CLC considering only reads with a minimum length of 180 bp and contigs no less than 200 bp long resulted in 634,772 and 788,464 unigenes (unique contigs), respectively. © 2020, The Author(s).

Журнал: Scientific Data

Выпуск журнала: Vol. 7, Is. 1

Номера страниц: 239

ISSN журнала: 20524463

• Breidenbach Natalie (Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany)
• Sharov Vadim V. (Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Lab Genom Res & Biotechnol,Fed Res Ctr, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Space & Informat Technol, Dept High Performance Comp, Krasnoyarsk 660074, Russia)
• Gailing Oliver (Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany; George August Univ Gottingen, Ctr Integrated Breeding Res, D-37075 Gottingen, Germany)
• Krutovsky Konstantin V. (Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany; George August Univ Gottingen, Ctr Integrated Breeding Res, D-37075 Gottingen, Germany; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Genome Res & Educ Ctr, Lab Forest Genom, Krasnoyarsk 660036, Russia; Russian Acad Sci, NI Vavilov Inst Gen Genet, Lab Populat Genet, Moscow 119333, Russia)

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• Web of Science Core Collection