Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.3390/ijms21228464
Ключевые слова: bacterial luciferase, enzymes, fluorescent protein, hormesis, low-dose radiation, oxidoreductase, tritium
Аннотация: The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (
Издание
Журнал: International Journal of Molecular Sciences
Выпуск журнала: Vol. 21, Is. 22
Номера страниц: 1-15
ISSN журнала: 16616596
Издатель: MDPI AG
Персоны
- Rozhko Tatiana V. (Krasnoyarsk State Med Acad, Dept Med & Biol Phys, Krasnoyarsk 660022, Russia)
- Nemtseva Elena V. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia; RAS, Inst Biophys, SB, FRC,KSC, Krasnoyarsk 660036, Russia)
- Gardt Maria V. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
- Raikov Alexander V. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
- Lisitsa Albert E. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
- Badun Gennadii A. (Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia)
- Kudryasheva Nadezhda S. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia; RAS, Inst Biophys, SB, FRC,KSC, Krasnoyarsk 660036, Russia)
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