Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria

Тип публикации: статья из журнала

Год издания: 2010

Идентификатор DOI: 10.1016/j.polymdegradstab.2010.08.023

Ключевые слова: Polyhydroxyalkanoates, PHA, PHA properties, Degradation of polymers, Biopolymers in marine environment, PHA-degrading microorganisms, Biopolymers in marine environment, Degradation of polymers, PHA properties, PHA-degrading microorganisms, Polyhydroxyalkanoates, PHA, 16S rRNA, 3-hydroxybutyric acid, Amorphous phase, Bacillus sp, Biodegradation rate, Chemical compositions, Coastal waters, Degradation rate, Degrading bacteria, Degree of crystallinity, Enterobacter, Hydroxyvalerate, Marine environment, PHA properties, PHA-degrading microorganisms, Polydispersity indices, Polyhydroxyalkanoates, Polymer chains, Preparation technique, Sole carbon source, South China Sea, Viet Nam, Acids, Bacteriology, Biodegradable polymers, Biodegradation, Biomolecules, Biopolymers, Microorganisms, Organic polymers, Polydispersity, Polymer films, Polymers, RNA, Seawater, Degradation

Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens: it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp. (C) 2010 Elsevier Ltd. All rights reserved.

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Издание

Журнал: POLYMER DEGRADATION AND STABILITY

Выпуск журнала: Vol. 95, Is. 12

Номера страниц: 2350-2359

ISSN журнала: 01413910

Место издания: OXFORD

Издатель: ELSEVIER SCI LTD

Авторы

  • Volova T.G. (Siberian Federal University)
  • Rudnev V.P. (Joint Russian-Vietnam Tropical Research and Test Centre,Coastal Branch)
  • Ba Xuan B. (Joint Russian-Vietnam Tropical Research and Test Centre,Coastal Branch)
  • Vit Dung V. (Joint Russian-Vietnam Tropical Research and Test Centre,Coastal Branch)
  • Boyandin A.N. (Siberian Federal University)
  • Gitelson I.I. (Institute of Biophysics,Siberian Branch,Russian Academy of Sciences)
  • Prudnikova S.V. (Siberian Federal University)
  • Vasiliev A.D. (L.V. Kirensky Institute of Physics,Siberian Branch,Russian Academy of Sciences)
  • Karpov V.A. (A.N. Severtsov Institute of Ecology and Evolution RAS)
  • Mishukova O.V. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
  • Boyarskikh U.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
  • Filipenko M.L. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)

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