An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation

Тип публикации: статья из журнала

Год издания: 2012

Ключевые слова: Degradable polymer matrices, Degradation, PHAs, Polyhydroxyalkanoates, Subcutaneous implantation, Tissue reaction

Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.

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Журнал: Cellular Transplantation and Tissue Engineering

Выпуск журнала: Vol. 7, Is. 1

Номера страниц: 73-80


  • Shishatskaya E.I. (Institute of Modern Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Nikolaeva E.D. (Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation)
  • Goreva A.V. (Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation)
  • Brigham C.J. (Massachusetts Institute of Technology, Cambridge, United States)
  • Volova T.G. (Institute of Modern Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Sinskey A.J. (Massachusetts Institute of Technology, Cambridge, United States)

Вхождение в базы данных

  • Scopus (цитирований 2)

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