Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1016/j.ijbiomac.2020.07.095

Ключевые слова: cupriavidus necator b-10646, emulsifiers, fatty acids, plant oils, polyhydroxyalkanoates

Аннотация: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate was palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9–1.9 mol%) and 3-hydroxyhexanoate (0.5–1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier. © 2020

Журнал: International Journal of Biological Macromolecules

Выпуск журнала: Vol. 164

Номера страниц: 121-130

ISSN журнала: 01418130

• Volova Tatiana (Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia; Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akad Gorodok, Krasnoyarsk 660036, Russia)
• Sapozhnikova Kristina (Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia; Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akad Gorodok, Krasnoyarsk 660036, Russia)
• Zhila Natalia (Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia; Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, 50-50 Akad Gorodok, Krasnoyarsk 660036, Russia)

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