Strategy for non-target ionic analysis by capillary electrophoresis with ultraviolet detection

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1007/s00216-016-0025-8

Ключевые слова: Capillary electrophoresis, Optimization of separation, Non-target analysis, Identification, Selectivity, Peak width

Аннотация: A strategy for non-target analysis of samples with unknown composition by capillary electrophoresis (CE) with ultraviolet (UV) detection is suggested. The strategy is based on the preliminary identification of analytes and further optimization of the conditions for their separation using the developed computational tool set ElphoSeparation. It is shown that, in order to record electrophoretic peaks with the mobilities from the maximum to minimum possible values, the positive and negative voltage polarity and hydrodynamic pressure should be used. To choose the optimal separation conditions, dynamic maps of electrophoretic separation (DMES) are suggested. DMES is a bar chart with theoretical resolutions of adjacent peaks presented in ascending order of the migration time. The resolution is calculated as the division of the difference of the effective electrophoretic mobilities of adjacent analytes by their average peak width in terms of electrophoretic mobility. The suggested strategy is tested by the example of the analysis of herbal medicine (Holosas) on the basis of rose hips. The approach should be used to analyze samples with not very complex composition, such as environmental water and precipitation samples, process liquors, some vegetable extracts, biological fluids, food, and other samples for the determination of widespread compounds capable of forming ionic species. For samples with complex composition, the approach used together with other techniques may produce advantageous information due to specificity of the method, particularly it can be useful for determination of compounds suffering from low volatility or thermal stability, and for analysis of samples with difficult matrices.

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Издание

Журнал: ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Выпуск журнала: Vol. 409, Is. 4

Номера страниц: 1067-1077

ISSN журнала: 16182642

Место издания: HEIDELBERG

Издатель: SPRINGER HEIDELBERG

Авторы

  • Sursyakova Viktoria V. (Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Akademgorodok 50-24, Krasnoyarsk 660036, Russia)
  • Burmakina Galina V. (Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Akademgorodok 50-24, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Svobodny Pr 79, Krasnoyarsk 660041, Russia)
  • Rubaylo Anatoly I. (Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Akademgorodok 50-24, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Svobodny Pr 79, Krasnoyarsk 660041, Russia; Russian Acad Sci, Krasnoyarsk Sci Ctr, Siberian Branch, Akademgorodok 50, Krasnoyarsk 660036, Russia)

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