Bioluminescent enzymatic rapid assay of water integral toxicity

Тип публикации: статья из журнала

Год издания: 2013

Идентификатор DOI: 10.1007/s10661-012-2994-1

Ключевые слова: Integral water toxicity, Ecological monitoring, Bioluminescence, Luciferase., Immobilization, Bioluminescence, Ecological monitoring, Immobilization, Integral water toxicity, Luciferase, Ecological monitoring, Enzymatic system, Field conditions, Luciferase, Maximum Efficiency, Maximum permissible concentration, Reaction catalyzed, Toxic substances, Chemical reactions, Heavy metals, Phenols, Radioactive waste vitrification, Toxicity, Water pollution, Bioluminescence, benzoquinone, cadmium sulfate, catechol, cobalt sulfate, copper sulfate, flavine mononucleotide reductase, hydroquinone, luciferase, methylbenzoquinone, naphthoquinone, quinone derivative, reduced nicotinamide adenine dinucleotide dehydrogenase, thymoquinone, unclassified drug, assay, concentration (composition), immobilization, organic pollutant, pulp and paper industry, toxicity, wastewater, water pollution, article, bioluminescence, concentration (parameters), ecotoxicity, enzyme assay, enzyme immobilization, pollution monitoring, process development, sensitivity and specificity, toxicity testing, waste water, water analysis, water contamination, water pollution control, water sampling, Environmental Monitoring, FMN Reductase, Luciferases, Luminescent Measurements, NAD, Water Pollutants, Chemical

Аннотация: A bioluminescent rapid method was developed to estimate the integral toxicity of natural and wastewater. This method is based on registering the effect of the polluted water sample on the parameters of the bioluminescent reaction catalyzed by the multi-component reagent containing NADH:FMN oxidoreductase, luciferase, and their substrates co-immobilized in a starch carrier. Several ways to increase the method's sensitivity to toxic substances were suggested; conditions were selected to make it possible to determine, with maximum efficiency, the content of toxic substances corresponding to a certain maximum permissible concentration. The sensitivity of soluble and immobilized coupled enzymatic systems to a series of organic pollutants (phenols, quinones, and salts of heavy metals) was compared. It was shown that the reagent is the most sensitive to the effect of phenols and quinones. The method was tested during analysis of the wastewater from a pulp and paper plant and can be used for biotesting in both laboratory and field conditions.

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Издание

Журнал: ENVIRONMENTAL MONITORING AND ASSESSMENT

Выпуск журнала: Vol. 185, Is. 7

Номера страниц: 5909-5916

ISSN журнала: 01676369

Место издания: DORDRECHT

Издатель: SPRINGER

Авторы

  • Esimbekova E.N. (Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)
  • Kondik A.M. (Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)
  • Kratasyuk V.A. (Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)

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