Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2013

Идентификатор DOI: 10.1016/j.biortech.2013.07.070

Ключевые слова: Cupriavidus eutrophus, Polyhydroxyalkanoates, Autotrophic synthesis, 3-Hydroxyhexanoate, Hydrogen-oxidizing bacteria, Nitrogen deficiency, Oxygen mass transfer, Physicomechanical properties, Poly-3-hydroxybutyrate, Nitrogen, Substrates, Carbon dioxide, 3 hydroxybutyric acid, 4 hydroxybutyric acid, gamma butyrolactone, hexanoic acid, hydrogen, manganese, phosphorus, poly(3 hydroxybutyric acid), polyhydroxyalkanoic acid, potassium, sulfur, valeric acid, autotrophy, bacterium, cell organelle, chemostat, mass transfer, physicochemical property, polymer, substrate, article, bacterial growth, bacterium culture, cell density, cell growth, concentration (parameters), Cupriavidus, flow rate, nonhuman, precursor, priority journal, synthesis, 3-Hydroxybutyric Acid, Biodegradation, Environmental, Bioreactors, Carbon, Clostridium beijerinckii, Crystallization, Fermentation, Gases, Oxygen, Polymers, Refuse Disposal, Temperature

Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D = 0.1 h(-1), on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460 h(-1), P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50 g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, gamma-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. (c) 2013 Elsevier Ltd. All rights reserved.

Журнал: BIORESOURCE TECHNOLOGY

Выпуск журнала: Vol. 146

Номера страниц: 215-222

ISSN журнала: 09608524

Место издания: OXFORD

Издатель: ELSEVIER SCI LTD

• Volova T.G. (Siberian Federal University)
• Peterson I.V. (Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences)
• Kiselev E.G. (Institute of Biophysics of Siberian Branch of Russian Academy of Sciences)
• Shishatskaya E.I. (Siberian Federal University)
• Zhila N.O. (Institute of Biophysics of Siberian Branch of Russian Academy of Sciences)
• Boyandin A.N. (Institute of Biophysics of Siberian Branch of Russian Academy of Sciences)
• Kalacheva G.S. (Institute of Biophysics of Siberian Branch of Russian Academy of Sciences)
• Syrvacheva D.A. (Siberian Federal University)
• Vinogradova O.N. (Siberian Federal University)
• Vasiliev A.D. (Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences)

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