On the mechanism of biological activation by tritium

Тип публикации: статья из журнала

Год издания: 2016

Идентификатор DOI: 10.1016/j.jenvrad.2016.03.017

Ключевые слова: Tritium, Low-dose effect, Radiation hormesis, Luminous marine bacteria, DNA mutations, Bacteria, Bioassay, Bioluminescence, Chemical activation, Luminescence, Phosphorescence, Physiological models, Radiation chemistry, Beta-emitting radionuclides, DNA mutation, Low dose effects, Luminescence intensity, Marine bacterium, Physiological parameters, Specific radioactivity, reactive oxygen metabolite, tritium oxide, bacterium, biological method, cells and cell components, dose-response relationship, ionization, marine environment, mutation, Article, bacterial mutation, biological activity, cell membrane, electrophoresis, gene mutation, nonhuman, Photobacterium phosphoreum, radioactivity, radiolysis, restriction fragment, Bacteria (microorganisms)

Аннотация: The mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface (tritium-labeled films, 0.11, 0.28, 0.91, and 236 MBq/cm(2)). When using the tritium-labeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescence kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans membrane cellular processes stimulated by ionization and radiolysis of aqueous media. (C) 2016 Elsevier Ltd. All rights reserved.

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Выпуск журнала: Vol. 157

Номера страниц: 131-135

ISSN журнала: 0265931X

Место издания: OXFORD



  • Rozhko T.V. (Siberian Federal University)
  • Guseynov O.A. (Siberian Federal University)
  • Guseynova V.E. (Siberian Federal University)
  • Kudryasheva N.S. (Institute of Biophysics SB RAS)
  • Badun G.A. (Moscow State University,Department of Chemistry)
  • Razzhivina I.A. (Moscow State University,Department of Chemistry)

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