Disposable luciferase-based microfluidic chip for rapid assay of water pollution

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1002/bio.3508

Ключевые слова: bioassay, lab-on-a-chip, luciferase, microfluidics, solvent bonding

Аннотация: In the present study, we demonstrate the use of a disposable luciferase-based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3-dihydroxybenzene and 1,4-benzoquinone for the proposed microfluidic biosensor measured 3 μM, 15 mM, and 2 μM respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using a gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers. © 2018 John Wiley & Sons, Ltd.

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Издание

Журнал: Luminescence

Выпуск журнала: Vol. 33, Is. 6

Номера страниц: 1054-1061

ISSN журнала: 15227235

Издатель: John Wiley and Sons Ltd

Авторы

  • Denisov I. (Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Lukyanenko K. (Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Yakimov A. (Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Kukhtevich I. (Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Esimbekova E. (Siberian Federal University, Krasnoyarsk, Russian Federation, Institute of Biophysics SB RAS Federal Research Center'Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, Russian Federation)
  • Belobrov P. (Siberian Federal University, Krasnoyarsk, Russian Federation)

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