Obelin mutants as reporters in bioluminescent dual-analyte binding assay

Тип публикации: статья из журнала

Год издания: 2013

Идентификатор DOI: 10.1039/c2ay25976a

Ключевые слова: Gene encoding, Allelic variants, Analytes, Binding assays, Factor V Leiden, Genotyping, Good correlations, High-throughput, Number of samples, Simultaneous detection, Single nucleotide polymorphisms, Single well, Time resolution, Traditional techniques, Bioluminescence

Аннотация: Two obelin mutants distinctly different in the color of bioluminescence were successfully applied to simultaneous detection of two analytes in a single well. The flash-type signals were triggered by single injection of Ca2+ and were discriminated by the spectral and time resolutions. The technique was developed in a high-throughput format, and applied to simultaneous immunoassay of two gonadotropic hormones - luteinizing (lutropin or hLH) and follicle stimulating (hFSH) - in clinical sera, and to simultaneous detection of two gene allelic variants at single nucleotide polymorphism genotyping of the human F5 gene encoding factor V Leiden polymorphism 1691 G -> A (R506Q). A considerable number of samples were investigated and the results obtained were in good correlation with those obtained by using traditional techniques.

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Издание

Журнал: ANALYTICAL METHODS

Выпуск журнала: Vol. 5, Is. 3

Номера страниц: 636-640

ISSN журнала: 17599660

Место издания: CAMBRIDGE

Издатель: ROYAL SOC CHEMISTRY

Авторы

  • Krasitskaya Vasilisa V. (Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia)
  • Kudryavtsev Alexander N. (Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia)
  • Shimomura Osamu (Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia; Marine Biol Lab, Woods Hole, MA 02543 USA)
  • Frank Ludmila A. (Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia)

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