Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.3390/nano10081499

Ключевые слова: bacterial assay, bioluminescence, enzymatic assay, humic acids-coated magnetite nanoparticles, hydrodynamic diameter, luciferase, magnetite nanoparticles, nadh:fmn-oxidoreductase, oxidative stress, photobacterium phosphoreum, silica-coated magnetite nanoparticles, toxicity, zeta potential

Аннотация: Current paper presents biological effects of magnetite nanoparticles (MNPs). Analyzing effects of MNP’ characteristics (zeta-potential and hydrodynamic diameters) on bacteria and their enzyme reactions was the main focus. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Журнал: Nanomaterials

Выпуск журнала: Vol. 10, Is. 8

Номера страниц: 1-20

ISSN журнала: 20794991

• Bondarenko Lyubov S. (Natl Res Univ, Moscow Aviat Inst, Moscow 125993, Russia)
• Kovel Ekaterina S. (FRC KSC SB RAS, Inst Phys SB RAS, Krasnoyarsk 660036, Russia; FRC KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia)
• Kydralieva Kamila A. (Natl Res Univ, Moscow Aviat Inst, Moscow 125993, Russia)
• Dzhardimalieva Gulzhian (Natl Res Univ, Moscow Aviat Inst, Moscow 125993, Russia; RAS, Moscow Inst Problems Chem Phys, Chernogolovka 142432, Moscow Region, Russia)
• Illes Erzsebet (Univ Szeged, H-6720 Szeged, Hungary)
• Tombacz Etelka (Univ Szeged, H-6720 Szeged, Hungary)
• Kicheeva Arina G. (Siberian Fed Univ, Krasnoyarsk 660041, Russia)
• Kudryasheva Nadezhda S. (FRC KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Krasnoyarsk 660041, Russia)

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