Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1002/elps.201900347

Ключевые слова: Betulin derivatives, Binding constants, Haarhoff-Van der Linde function, High-affinity interaction, Inclusion complexes, inclusion complexesbetulin, 473-98-3, gamma cyclodextrin, 17465-86-0

Аннотация: The complexation between (2-hydroxypropyl)-gamma-cyclodextrin (HP-gamma-CD) and water-soluble betulin derivatives, betulin 3,28-disulfate (DSB) and betulin 3-acetate-28-sulfate (ASB), belonging to the class of pentacyclic lupane triterpenoids, was studied using mobility shift ACE (ms ACE). It was found that the complexation is a high-affinity interaction. In this case, a very low amount of HP-gamma-CD should be added to the BGE, and triangular peaks are observed as a result of ligand deficiency in the sample zone. Le Saux et al. showed in 2005 that using the parameter a(1) of the Haarhoff-Van der Linde (HVL) function instead of the migration time measured at the peak apex eliminates the effect of ligand deficiency on effective electrophoretic mobility. Therefore, the electrophoretic mobilities of asymmetrical peaks of DSB and ASB were calculated in this way. The obtained experimental data correspond to 1:1 complexes. The calculated values of binding constants logarithms at 25 degrees C are 6.70 +/- 0.05 and 7.03 +/- 0.10 for the HP-gamma-CD complexes of DSB and ASB, respectively.

Журнал: ELECTROPHORESIS

Выпуск журнала: Vol. 41, 1-2

Номера страниц: 112-115

ISSN журнала: 01730835

Место издания: HOBOKEN

Издатель: WILEY

• Sursyakova Viktoria V. (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia)
• Levdansky Vladimir A. (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia)
• Rubaylo Anatoly (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Krasnoyarsk, Russia; Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Krasnoyarsk, Russia)

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