Сибирский федеральный университет

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.3390/ijms22126382

Ключевые слова: fullerenol, toxicity, antioxidant activity, reactive oxygen species, bioluminescent assay, hormesis

Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene's carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.

Журнал: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Выпуск журнала: Vol. 22, Is. 12

Номера страниц: 6382

ISSN журнала: 14220067

Место издания: BASEL

Издатель: MDPI

• Kovel Ekaterina S. (FRC KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; FRC KSC SB RAS, Inst Phys SB RAS, Krasnoyarsk 660036, Russia)
• Kicheeva Arina G. (FRC KSC SB RAS, Krasnoyarsk 660036, Russia)
• Vnukova Natalia G. (FRC KSC SB RAS, Inst Phys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)
• Churilov Grigory N. (FRC KSC SB RAS, Inst Phys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)
• Stepin Evsei A. (Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)
• Kudryasheva Nadezhda S. (FRC KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)

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