Gene-specific sex-linked genetic markers in date palm (Phoenix dactylifera L.) : научное издание

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1007/s10722-017-0564-7

Ключевые слова: Date palm, Dioecious, Genetic markers, Haplotypes, Hemizygous, Heterogametic, Monoecious, Phoenix dactylifera, Sex genes, SNPs

Аннотация: During the past decade, there have been numerous attempts to identify sex-linked molecular genetic markers that can be used to discriminate among male and female trees in date palm (Phoenix dactylifera L.). In our approach to address this biological problem, we applied a comparative genomics approach and used a candidate sex-linked Tormozembryo Defective (TOZ19) gene found to be male-specific in aspen. Using BLAST against the date palm genome assembly, we found a putative Transducin Beta-like Protein 3 (TBL3) gene in date palm that was highly homologous to the TOZ19 gene and sequenced it in three male and four female trees from four economically important date palm cultivars from Egypt. Based on the obtained multiple nucleotide sequence alignments, male- and female-specific date palm haplotypes were identified by screening single nucleotide polymorphisms (SNPs). Subsequently, a respective gene fragment in additional five date palm samples comprising three females and two males were cloned and sequenced to independently confirm the previously identified putative sex-linked SNPs. The three putative sex-linked SNPs can be used now to discriminate male and female date palms at their seedling stage. This will further enhance and pave the way for commercial date palm cultivation through seeds. The identified molecular markers are relatively easy, cheap, fast, and reproducible sex identification tools. Female date palms are either homozygous or heterozygous, while male date palms are hemizygous at the putative sex-linked loci. © 2017 Springer Science+Business Media B.V.

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Издание

Журнал: Genetic Resources and Crop Evolution

Номера страниц: 1-10

ISSN журнала: 09259864

Издатель: Springer Netherlands

Авторы

  • Ali H.B.M. (Department of Genetics and Cytology, National Research Centre, Cairo, Egypt, Department of Forest Genetics and Forest Tree Breeding, Georg-August University of Göttingen, Büsgenweg 2, Göttingen, Germany)
  • Abubakari A. (Department of Forest Genetics and Forest Tree Breeding, Georg-August University of Göttingen, Büsgenweg 2, Göttingen, Germany)
  • Wiehle M. (Centre for International Rural Development (Tropenzentrum), International Center for Development and Decent Work (ICDD), Faculty of Organic Agricultural Sciences, University of Kassel, Steinstraße 19, Witzenhausen, Germany)
  • Krutovsky K.V. (Department of Forest Genetics and Forest Tree Breeding, Georg-August University of Göttingen, Büsgenweg 2, Göttingen, Germany, N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina Str, Moscow, Russian Federation, Genome Research and Education Center, Siberian Federal University, 50a/2 Akademgorodok, Krasnoyarsk, Russian Federation, Department of Ecosystem Science and Management, Texas A&M University, 2138 TAMU, College Station, TX, United States)

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