Application of Enzyme Bioluminescence for Medical Diagnostics

Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций

Год издания: 2014

Идентификатор DOI: 10.1007/978-3-662-43385-0_6

Ключевые слова: Bioluminescence, Ca2+-regulated photoprotein, Diagnostics, Immunoassay, Luciferase, Nucleic acid hybridization assay, Bioluminescence, Ca2+-regulated photoprotein, Diagnostics, Immunoassay, Luciferase, Nucleic acid hybridization assay, enzyme, animal, diagnosis, human, immunoassay, luminescence, metabolism, nucleic acid hybridization, procedures, Animals, Diagnosis, Enzymes, Humans, Immunoassay, Luminescent Measurements, Nucleic Acid Hybridization

Аннотация: Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand for new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.

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Издание

Журнал: BIOLUMINESCENCE: FUNDAMENTALS AND APPLICATIONS IN BIOTECHNOLOGY, VOL 1

Выпуск журнала: Vol. 144

Номера страниц: 175-197

ISSN журнала: 07246145

Место издания: BERLIN

Издатель: SPRINGER-VERLAG BERLIN

Авторы

  • Frank L.A. (Siberian Federal University)
  • Krasitskaya V.V. (Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch)

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