RNA Aptamer against Autoantibodies Associated with Multiple Sclerosis and Bioluminescent Detection Probe on Its Basis

Тип публикации: статья из журнала

Год издания: 2014

Идентификатор DOI: 10.1021/ac4037894

Ключевые слова: Autoantibodies, Detection probes, Developed model, High affinity, Laboratory test, Microplate assay, Multiple sclerosis, Myelin basic protein, Antibodies, RNA, Bioluminescence, aptamer, autoantibody, molecular probe, adolescent, adult, female, human, immunology, luminescence, male, middle aged, molecular probe, multiple sclerosis, young adult, Adolescent, Adult, Aptamers, Nucleotide, Autoantibodies, Female, Humans, Luminescence, Male, Middle Aged, Molecular Probes, Multiple Sclerosis, Young Adult

Аннотация: Nowadays, there are no specific laboratory tests for establishing the diagnosis of multiple sclerosis (MS). The presence of proteolytic autoantibodies against myelin basic protein is now considered as a characteristic feature of MS. New 2'-F-containing RNA aptamer of high affinity and specificity to these antibodies was selected. Covalent conjugate of this aptamer and Ca2+-regulated photoprotein obelin was obtained for the first time and applied as a label in bioluminescent microplate assay to detect target antibodies. The developed model solid-phase microassay is simple, fast, and highly sensitive.

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Издание

Журнал: ANALYTICAL CHEMISTRY

Выпуск журнала: Vol. 86, Is. 5

Номера страниц: 2590-2594

ISSN журнала: 00032700

Место издания: WASHINGTON

Издатель: AMER CHEMICAL SOC

Авторы

  • Vorobjeva M.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
  • Fokina A.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
  • Timoshenko V.V. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
  • Nevinsky G.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
  • Venyaminova A.G. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
  • Krasitskaya V.V. (Siberian Federal University)
  • Frank L.A. (Siberian Federal University)

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