Тип публикации: статья из журнала
Год издания: 2014
Идентификатор DOI: 10.1021/ac4037894
Ключевые слова: Autoantibodies, Detection probes, Developed model, High affinity, Laboratory test, Microplate assay, Multiple sclerosis, Myelin basic protein, Antibodies, RNA, Bioluminescence, aptamer, autoantibody, molecular probe, adolescent, adult, female, human, immunology, luminescence, male, middle aged, young adult, Aptamers, Nucleotide, Humans, Molecular Probes
Аннотация: Nowadays, there are no specific laboratory tests for establishing the diagnosis of multiple sclerosis (MS). The presence of proteolytic autoantibodies against myelin basic protein is now considered as a characteristic feature of MS. New 2'-F-containing RNA aptamer of high affinity and specificity to these antibodies was selected. Covalent conjugate of this aptamer and Ca2+-regulated photoprotein obelin was obtained for the first time and applied as a label in bioluminescent microplate assay to detect target antibodies. The developed model solid-phase microassay is simple, fast, and highly sensitive.
Издание
Журнал: ANALYTICAL CHEMISTRY
Выпуск журнала: Vol. 86, Is. 5
Номера страниц: 2590-2594
ISSN журнала: 00032700
Место издания: WASHINGTON
Издатель: AMER CHEMICAL SOC
Персоны
- Vorobjeva M.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
- Fokina A.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
- Timoshenko V.V. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
- Nevinsky G.A. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
- Venyaminova A.G. (Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences)
- Krasitskaya V.V. (Siberian Federal University)
- Frank L.A. (Siberian Federal University)
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