System peaks in capillary zone electrophoresis of anions with negative voltage polarity and counter-electroosmotic flow

Тип публикации: статья из журнала

Год издания: 2011

Идентификатор DOI: 10.1002/elps.201000313

Ключевые слова: Anions, CE, Counter-EOF, Indirect detection, System peak, Anions, CE, Counter-EOF, Indirect detection, System peak, anion, analytical error, analytical parameters, analyzer, article, capillary zone electrophoresis, concentration (parameters), counter electroosmotic flow, flow rate, hydrodynamics, mathematical model, negative voltage polarity, prediction, pressure, separation technique, system peak, Anions, Electroosmosis, Electrophoresis, Capillary, Mathematical Computing, Spectrophotometry

Аннотация: The system peaks that often appear on electropherograms in anion separation by CE with indirect spectrophotometric detection, negative voltage polarity and cathodic EOF are studied. The system peaks are shown to correspond to the zones with the changed concentration of the BGE constituents; they appear while the zone of each analyte anion passes through the outlet end of the capillary and are transported to the detector by EOF. An equation is suggested for predicting migration times of the system peaks with an error of 1%. The ratios of the system peak area to the analyte peak area are found to amount to 20%. It is shown that it is possible to avoid overlapping of the system peaks and analyte peaks by controlling the EOF velocity owing to hydrodynamic pressure. Using the mathematical simulation of CE shows that the system peaks and baseline shift can result from changing the transference numbers of the BGE ions and analyte ions at the capillary edge. The cases when the system peak may be incorrectly identified as the peak of analyte ion are considered. In order to avoid such errors, some practical recommendations are given.

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Издание

Журнал: ELECTROPHORESIS

Выпуск журнала: Vol. 32, Is. 2

Номера страниц: 210-217

ISSN журнала: 01730835

Место издания: WEINHEIM

Издатель: WILEY-V C H VERLAG GMBH

Авторы

  • Sursyakova V.V. (Institute of Chemistry and Chemical Technology,Siberian Branch of the Russian Academy of Sciences)
  • Kalyakin S.N. (Institute of Chemistry and Chemical Technology,Siberian Branch of the Russian Academy of Sciences)
  • Burmakina G.V. (Institute of Chemistry and Chemical Technology,Siberian Branch of the Russian Academy of Sciences)
  • Rubaylo A.I. (Siberian Federal University)

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