Novel spray-dried PHA microparticles for antitumor drug release

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1080/07373937.2017.1407940

Ключевые слова: 5-Fluorouracil, efficiency, microparticles, paclitaxel, polyhydroxyalkanoates, spray-drying

Аннотация: The production of poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (PEG)-based microparticles, loaded with antitumor drugs paclitaxel (PTX) and 5-Fluorouracil (5-FU) by spray-drying technique, was investigated. The average diameter of microparticles was found to be 3.4 +/- 0.5 pm and zeta potential was about -44 mV. The addition of surfactant PEG did not show any effect on the morphological characteristics of the particles. But the chemical structure of drug influenced on the properties. Microparticles had heterogeneous pores on the surface when the hydrophobic PTX was encapsulated. It was established that the addition of surfactant positively influenced on the properties of particles and led to the loading of 5-FU directly into the matrix. This is confirmed by the results of electron microscopy and dynamics of drug release in vitro. As a whole, the release profiles of PTX and 5-FU from composite P3HB/PEG microparticles were less than from P3HB microparticles. The results of the morphological evaluation of Hela cells demonstrated that the use of cytostatic drugs loaded in P3HB microparticles induces morphological changes associated with apoptosis (chromatin condensation, core fragmentation, margination of nucleus). Thus, the obtained results can serve as the basis for the development of new antitumor drugs of prolonged action, intended for various modes of administration.

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Издание

Журнал: DRYING TECHNOLOGY

Выпуск журнала: Vol. 36, Is. 11

Номера страниц: 1387-1398

ISSN журнала: 07373937

Место издания: PHILADELPHIA

Издатель: TAYLOR & FRANCIS INC

Авторы

  • Shershneva Anna (Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, 79 Svobodny, Krasnoyarsk 660041, Russia)
  • Murueva Anastasiya (SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, Tomsk, Russia)
  • Nikolaeva Elena (SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, Tomsk, Russia)
  • Shishatskaya Ekaterina (Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, 79 Svobodny, Krasnoyarsk 660041, Russia; SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, Tomsk, Russia)
  • Volova Tatiana (Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, 79 Svobodny, Krasnoyarsk 660041, Russia; SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, Tomsk, Russia)

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