Bioluminescent enzyme assay for the indication of plant stress in enclosed life support systems

Тип публикации: статья из журнала

Год издания: 2011

Идентификатор DOI: 10.1002/bio.1267

Ключевые слова: bioluminescence, enclosed environmental chambers, luciferase, pyridine nucleotides, stress in plants, bioluminescence, enclosed environmental chambers, luciferase, pyridine nucleotides, stress in plants, nicotinamide adenine dinucleotide phosphate, article, luminescence, metabolism, physiological stress, physiology, Raphanus, Luminescence, NADP, Raphanus, Stress, Physiological

Аннотация: The application of bioluminescent sensors for monitoring key metabolites and enzymes that are indicators of stress in plants is demonstrated. The sensitivity of bioluminescent assay for NAD(P)H and NAD(P)(+) was about 0.5 and 1 nmol, respectively. The levels of NAD(P)H and NAD(P)(+) in radish (Raphanus sativus) root extracts from controls and from stress-induced conditions were compared. To induce environmental stress, the plants were grown in enclosed environmental chambers with low pressure (9 or 32 kPa), high humidity (>80%) and low oxygen partial pressure (down to 3.3-6.5 kPa). The concentrations of NAD(P)(+) and NAD(P)H in plants varied under stress conditions. Decreasing both total pressure from 101.5 to 32 or 9 kPa and partial pressure of oxygen increased the ratio of NAD(P)(+)/NAD(P)H from 0.2 to 4 or 6, respectively. The increase in this ratio suggests that plants are undergoing stress in these hypobaric environments. The developed bioluminescent assay for quantification of pyridine nucleotides in plant tissues is rapid, low-cost and easily performed. Copyright (C) 2011 John Wiley & Sons, Ltd.

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Издание

Журнал: LUMINESCENCE

Выпуск журнала: Vol. 26, Is. 6

Номера страниц: 543-546

ISSN журнала: 15227235

Место издания: MALDEN

Издатель: WILEY-BLACKWELL

Авторы

  • Kratasyuk V. (Institute of Biophysics,SB RAS)
  • Esimbekova E. (Institute of Biophysics,SB RAS)
  • Correll M. (Agricultural and Biological Engineering Department,University of Florida)
  • Bucklin R. (Agricultural and Biological Engineering Department,University of Florida)

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